针灸行为中医瑰宝，历经数千年传承，在编削东说念主体机能、防治疾病方面彰显出专有上风。跟着当代医学技能的发展，针灸的临床价值与科学机制不停被挖掘，在祸患看护、代谢编削、神经康复等多个规模展现出宽广的应用远景，为治理应代疑难病症提供了全新的调养念念路。本期为全球保举发表于《针刺策动》《中国针灸》的 6 篇双语著述，它们从表面默契、机制探索、临床考据到技能翻新，全标的展现了针灸学科的当代化发展效果。真挚邀请全球深化研读这些优质效果，一同探索针灸的科学高明，见证传统奢睿与当代医学的碰撞交融，为关连规模的策动与实践注入新的活力！

精选著述Selected Articles

01

论针灸的双向编削效应

On the bidirectional regulation effect of acupuncture

【摘抄】中医爱戴健康的奢睿是“阴平阳秘”。不言而谕，“稳态”守护存在“过之”与“不足”的双重身分，人命经过必须有“双向编削”系统的维稳机制来护卫。因此，守护“稳态”均衡的双向调控波及“矛”与“盾”相对零丁的两边势能，还需依靠两套相对零丁的调控系统智力完成。针灸是人命体清晰“双向编削效应”的优等担当者，但针灸的这种效应是否能由单一穴位激发？这本不是问题的问题却成了针灸学科的一大问题。针灸“双向编削”主见的过度拓展，中医表面难以如斯兼顾，只不外是私自扩大了表面外延的规模。本文从人命科学的角度弘扬：针灸的“双向”效应主淌若通过躯壳不同分区部位的穴位分传说入，通过两套相对零丁的调控系统，以守护人命“稳态”均衡的双向调控。

【Abstract】The wisdom of Chinese medicine in maintaining health is "Yin Ping Yang Mi" (balance between yin and yang). Obviously, the maintenance of "homeostasis" contains dual factors of "excessiveness" and "falling short". The life process must be protected by the stability-maintaining mechanism of the "bidirectional regulation" system. Therefore, the two-way regulation of the "homeostasis" maintaining involves the relatively independent potential energy of the "spear" and "shield", and also depends on two sets of relatively independent regulatory systems to complete. Acupuncture is an excellent performer of the "bidirectional regulation effect" of living organisms, but can this effect be caused by a single acupoint stimulation? This issue, which was not a problem, has become a big problem in acupuncture-moxibustion discipline. The concept of "two-way regulation" of acupuncture is over-expanded, which is difficult for the theory of Chinese medicine to take into account. It is only a broad expansion of the boundary of theoretical extension arbitrarily. From the perspective of life science, the present paper expounds that the "bidirectional regulation effect" of acupuncture is obtained from the activation of the "integration center" (not necessarily limited to thebrain) by the stimulated acupoints at different somatic parts of the body, and through two sets of relatively independentregulation systems, so as to maintain the life's "homeostasis”.

02

不同强度电针对内脏痛大鼠的镇痛作用

Analgesic effects of electroacupuncture at different intensities on visceral pain in rats

【摘抄】方针：不雅察不同强度电针对内脏痛大鼠的镇痛作用，并商量其脊髓上核心机制和量效关系。设施：雄性SD大鼠飞快分为普通组、模子组、不同强度（0.5、1、2、3、6 mA）电针组，每组6只。给与芥子油灌肠法建造内脏痛大鼠模子。纪录每组6只大鼠腹直肌肌电不雅察大鼠内脏通顺反射：60 mm Hg直结肠延迟刺激（CRD）诱发各组大鼠腹直肌放电20 s，随后赐与“足三里”1 min不同强度的电针干涉（强度分别为0.5、1、2、3、6 mA，波宽为0.5 ms，频率为10 Hz），电针达成后再次进行20 s CRD，比较电针前后CRD诱发腹直肌肌电的弧线底下积。另取48只SD大鼠飞快分为模子组及0.5、1、2、3、6 mA电针组，每组8只，多通说念在体电生理技能纪录模子组大鼠结直肠灌输芥子油后1 h延髓背侧网状亚核（SRD）神经元电行为的变化，各电针组在灌输芥子油10 min后赐与1 min电针，不雅察比较不同强度电针前后SRD神经元伤害性电行为放电频率的改变，统计电针后放电频率减少跨越20%的SRD神经元的比例。胁制：与普通组比较，干涉前模子组和不同强度电针组60 mm Hg CRD诱发的腹直肌肌电弧线底下积显赫增多（P<0.001）；干涉后与模子组比较，高强度（2、3、6 mA）电针组大鼠诱发的肌电弧线底下积显赫减少（P<0.05，P<0.001）；与低强度（0.5 mA和1 mA）电针组比较，高强度（2、3、6 mA）电针组大鼠诱发的腹直肌肌电弧线底下积显赫减少（P<0.01，P<0.001，P<0.05）。与造模前比较，灌输芥子油后大鼠SRD神经元电行为显赫增多，在5～30 min能守护欢叫且处于相对沉静景况（P<0.05）；在第11分钟赐与高强度（2、3、6 mA）电针后大鼠SRD神经元放电频率较电针前显赫质问（P<0.01，P<0.001）；与0.5、1 mA比拟，2、3、6 mA电针后神经元放电频率质问的差值不同进度增多（P<0.05，P<0.01，P<0.001），电针后的频率差值、神经元放电频率减少20%以上神经元的比例均跟着电针强度的增多而升高。论断：高强度电针约略缓解大鼠内脏痛，阻挠大鼠SRD神经元伤害性电行为，且阻挠效果在一定范围内随电针强度增多而增强。

【Abstract】Objective To observe the analgesic effect of electroacupuncture (EA) at different intensities on visceral pain in rats and explore the supraspinal central mechanism and dose-response relationship. Methods Male SD rats were randomly divided into anormal group, a model group and the groups of EA at different intensities (0.5, 1, 2, 3 and 6 mA, 6 rats/group). The visceral pain rat model was established using mustard oil enema. Abdominal rectus electromyography (EMG) was used to observe visceral motor response in the rats. A colorectal distension (CRD) of 60 mm Hg was adopted to induce abdominal rectus muscle discharge for 20 s in each group. Afterwards, EA (0.5, 1, 2, 3, and 6 mA of intensity, 0.5 ms of pulse width, 10 Hz of frequency) was employed at “Zusanli” (ST 36) for 1 min. After the end of EA, CRD was performed again for 20 s. Before and after EA, the areas under the curve (AUC) of CRD-induced abdominal rectus EMG were compared. Another set of rats was randomly divided into a model group and the groups of EA at different intensities (0.5, 1, 2, 3 and 6 mA, 8 rats/group). Multi-channel in vivo electrophysiological technique was used to record the changes in electric activity of the subnucleus reticularis dorsalis (SRD) neurons in the medulla oblongate of rats, and SRD neural nociceptive electrical activity was observed 1 h after rectal mustard oil infusion. EA at different intensities was administered for 1min at the 10-minute mark after rectal mustard oil infusion, and the changes in discharge frequency of nociceptive electrical activity of SRD neurons were compared before and after EA at different intensities. The proportion of SRD neurons whose discharge frequency decreased by more than 20% after EA was calculated. Results Compared to the normal group, the AUC of abdominal rectus EMG induced by 60mm Hg CRD was increased in the model group and EA of different intensity groups before intervention (P<0.001). After intervention, compared with the model group, AUC was decreased in the high-intensity (2, 3 and 6 mA) EA groups (P<0.05, P<0.001). When compared with the low-intensity (0.5 and 1 mA) EA groups, AUC was reduced in the high-intensity EA groups (P<0.01, P<0.001, P<0.05). In comparison with the indicators before modeling, the electrical activity of SRD neurons after rectal mustard oil infusion increased and remained excited and relatively stable in the duration from the 5-minute mark to the 30-minute mark in the rats (P<0.05); and after high-intensity EA at 11-minute mark, the discharge frequency of SRD neurons was reduced (P<0.01, P<0.001). Compared with the low-intensity EA, after the high-intensity EA, the reduced difference of discharge frequency increased (P<0.05, P<0.01, P<0.001). The frequency difference and the proportion of discharge frequency increasing over 20% after EA were elevated with the increase of EA intensity. Conclusion High-intensity EA can alleviate visceral pain and inhibit nociceptive electrical activity of SRD neurons in rats and the inhibitory effects are strengthened with the increase of EA intensity within a certain range.

03

针刺通督养心组方调养失眠症的东说念主工智能援手决议平台斥地策动

Development and research of an AI-assisted decision-making platform in treatment of insomnia with acupuncture of Tongdu Yangxin acupoint prescription

【摘抄】方针：构建并考据针刺通督养心组方调养失眠症的疗效展望模子，斥地灵通分享的交互式东说念主工智能（AI）援手决议平台。设施：纳入领受针刺通督养心组方调养的139例失眠症患者的临床贵府。患者均给与针刺通督养心组方调养，穴取百会、印堂、神门（双侧）和三阴交（双侧），百会和印堂畅达电针，给与连气儿波，频率2 Hz。隔日1次，每周3次，共调养2周。将匹兹堡寝息质料指数（PSQI）评分减分率<50%的患者归类为“无搪塞组”，PSQI评分减分率≥50%的患者归类为“搪塞组”。给与1.5倍四分位距端正进行相配值处理，并哄骗展望均值匹配法对缺失值进行多重插补。随后，中式顶点梯度擢升与飞快丛林算法特征病笃性胁制的杂乱行为模子的特搜集。在应用合成少数类过采样技能均衡类别后，辞别20%数据行为考据集，对剩尾数据通过固定比例飞快分层采样赢得200对3∶1的训练集与测试集，用于8种机器学习算法的模子训练与里面考据，进而筛选出用于构建最终模子的最好算法和数据集辞别心情，并进行外部考据。最终通过Streamlit将最好模子线上部署，构建交互式AI援手决议平台。胁制：用于模子构建的重要特征包括失眠病程、PSQI总分、PSQI寝息着力得分、N1期占总寝息时长比例、N2期占总寝息时长比例和寝息时间最高脉率。基于类别型特征擢升（CatBoost）算法构建的最好模子在测试集上的弧线底下积（AUC）为0.92，平均精准率为0.77，准确率、平均调回率、平均F1分数均为0.75；在考据集上的AUC为0.84，准确率、平均精准率、平均调回率、平均F1分数均为0.72，标明该模子具有雅致的展望性能。基于此模子斥地了交互式东说念主工智能援手决议平台（https://tdyx-catboost.streamlit.app/）。论断：本策动奏效构建并考据了基于CatBoost的针刺通督养心组方调养失眠症的疗效展望模子，所斥地的AI平台为针灸调养失眠症提供了数据驱动的援手决议复古。

【Abstract】Objective To construct and validate a predictive model for the therapeutic effect of acupuncture at Tongdu Yangxin prescription (acupoint prescription for promoting the circulation of the governor vessel and nourishing the heart) on insomnia, so as to develop an open-access interactive artificial intelligence (AI)-assisted decision-making platform. Methods Clinical data of 139 insomnia patients treated with Tongdu Yangxin acupuncture therapy were included. All the patients had received acupuncture at Baihui (GV 20), Yintang (GV24+), bilateral Shenmen (HT7), and bilateral Sanyinjiao (SP 6); and electric stimulation was attached to Baihui (GV20) and Yintang (GV24+), using a continuous wave and a frequency of 2 Hz. The treatment was delivered once every other day, 3 treatments a week, and for 2 consecutive weeks. Patients with Pittsburgh sleep quality index (PSQI) score reduction rate <50% were classified as the "no response group", and those with ≥50% were as the "response group". Outliers were addressed using the 1.5×IQR rule, and missing values were imputed via predictive mean matching. Key features were selected by intersecting the feature importance results from eXtreme Gradient Boosting (XGBoost) and random forest algorithms. After balancing class distribution using the Synthetic Minority Over-sampling Technique (SMOTE), 20% of the data was reserved as a validation set. The remained data underwent the stratified sampling iterations to generate 200 pairs of 3:1 training-test sets, which was employed for training and internal validation of 8 machine learning algorithms. The optimal algorithm and data partitioning strategy were selected to construct the final model, followed by external validation. The best-performing model was deployed online via Streamlit to create an interactive AI platform. Results Key predictive features for model construction included insomnia duration, the total PSQI score, PSQI sleep efficiency subscore, the proportion of N1 and N2 sleep stages in total sleep duration, and the maximum pulse rate during sleep. The CatBoost-based model achieved an AUC of 0.92, the average precision of 0.77, and accuracy, average recall, and average F1-score of 0.75 on the test set. On the validation set, it attained an AUC of 0.84, with accuracy, average precision, average recall, and average F1-score all at 0.72, demonstrating robust predictive performance. An interactive AI platform was subsequently developed (https://tdyx-catboost.streamlit.app/). Conclusion This study successfully establishes and validates a CatBoost-based efficacy prediction model for Tongdu Yangxin acupuncture therapy in treatment of insomnia. The developed AI platform provides driven decision support for acupuncture-based insomnia management.

04

基于实在寰宇的急性期针灸调养特发性面神经麻木：部队策动

Effect of acupuncture-moxibustion on idiopathic facial palsy at acute phase in the real world: a cohort study

【摘抄】方针：比较急性期和规复期介入针灸对特发性面神经麻木（IFP）的影响。设施：左证是否在急性期内领受针灸干涉将198例IFP患者分为早期针灸组（118例）和非早期针灸组（80例），经倾向性评分匹配（PSM），最终早期针灸组与非早期针灸组各纳入70例。在西医旧例调养基础上，两组均领受针灸调养，早期针灸组于急性期（病程≤7 d）介入针灸，非早期针灸组于规复期（病程>7 d）介入针灸。急性期予温针灸调养，穴取患侧翳风、下关、合谷、足三里；规复期予电针调养，穴取患侧攒竹、丝竹空、阳白等，予疏密波，频率2 Hz/100 Hz。均每次30 min，隔日1次，每周3次，共调养4周。比较两组患者调养前、调养1周和调养4周后House-Brackmann（H-B）面神经功能分级、Sunnybrook面神经功能评分和面部残疾指数（FDI）评分。并于调养1、4周后评定两组临床疗效和安全性。胁制：调养1、4周后，两组患者H-B面神经功能分级均较调养前改善（P<0.05）；调养4周后，早期针灸组H-B面神经功能分级优于非早期针灸组（P<0.05）。调养1、4周后，两组患者Sunnybrook面神经功能评分、躯体功能评定（FDIP）评分较调养前升高（P<0.05）；调养4周后，早期针灸组Sunnybrook面神经功能评分、FDIP评分升高幅度大于非早期针灸组（P<0.05）。调养4周后，两组社会生存功能评定（FDIS）评分较调养前质问（P<0.05）。调养4周后，早期针灸组总有用率（97.1%，68/70）高于非早期针灸组（87.1%，61/70，P<0.05）。两组不良事件发生率比较各异无统计学道理（P>0.05）。论断：在急性期介入针灸较规复期介入针灸更成心于IFP患者受损面神经功能康复。

【Abstract】Objective To compare the effect of acupuncture-moxibustion on idiopathic facial palsy (IFP) at acute phase and recovery phase. Methods According to whether received acupuncture-moxibustion at acute phase or not, 198 IFP patients were divided into an early-phase intervention group (118 cases) and anon-early-phase intervention group (80 cases). With the propensity score matching employed, 70 cases were included in each group. On the basis of the conventional treatment of western medicine, acupuncture-moxibustion was supplemented in the two groups. In the early-phase intervention group, acupuncture-moxibustion was delivered at the acute phase (duration of illness ≤7 days); in the non-early-phase intervention group, acupuncture-moxibustion was operated at the recovery phase (duration of illness > 7 days). At the acute phase, warm needling was performed at Yifeng (TE 17), Xiaguan (ST 7), Hegu (LI 4) and Zusanli (ST 36) on the affected side; and at the recovery phase, electroacupuncture was delivered at Cuanzhu (BL 2), Sizhukong (TE 23) and Yangbai (GB 14), etc. on the affected side, with the disperse-dense wave and 2 Hz/100 Hz of frequency. The intervention was operated for 30 min each time, once every two days, three treatments weekly and for 4 weeks. Before treatment, 1 week and 4 weeks of treatment, the House-Brackmann (H-B) facial nerve function grade, the score of Sunnybrook facial nerve function, and the score of facial disability index (FDI) were compared between the two groups. The clinical effect in 1 and 4 weeks of treatment and safety were evaluated. Results In 1 and 4 weeks of treatment, the H-B grade was improved when compared with that before treatment in each group (P<0.05), and in 4 weeks of treatment, H-B grade in the early-phase intervention group was superior to that of the non-early-phase intervention group (P<0.05). In 1 and 4 weeks of treatment, Sunnybrook scores and the scores of physical function of FDI were elevated in comparison with those before treatment in the two groups (P<0.05); and in 4 weeks of treatment, the elevation of these two indexes in the early-phase intervention group was greater than that of the non-early-phase intervention group (P<0.05). In 4 weeks of treatment, the scores of social function in FDI were reduced when compared with those before treatment in the two groups (P<0.05). In 4 weeks of treatment, the total effective rate (97.1%, 68/70) in the early-phase intervention group was higher than that (87.1%, 61/70) of the non-early-phase intervention group (P<0.05). There was no significant difference in the incidence of adverse events between the two groups (P>0.05). Conclusion Acupuncture-moxibustion therapy starting at the acute phase is more beneficial to the functional recovery of the impaired facial nerve than at the recovery phase in the IFP patients.

05

电针调控下丘脑LKB1/AMPK信号通路对痴肥小鼠白色脂肪棕色化的影响

Electroacupuncture intervention promotes browning of white adipose tissue by regulating LKB1/AMPK pathway in obese mice

【摘抄】方针：不雅察电针对痴肥小鼠下丘脑肝激酶B1（LKB1）/腺苷酸活化卵白激酶（AMPK）信号通路、交感神经活性及白色脂肪棕色化的调控作用，探索电针促进白色脂肪组织棕色化松开痴肥的核心机制。设施：C57BL/6J小鼠飞快分为普通组（8只）和造模组（30只），给与高脂饮食喂养建造痴肥小鼠模子。造模奏效的16只小鼠飞快分为模子组和电针组，每组各8只。电针组中式双侧“足三里”“天枢”电针调养，30 min/次，5次/周，共调养4周。不雅察调养前后各组小鼠体质料并谋略Lee’s指数；ELISA法测定空心血清甘油三酯（TG）、总胆固醇（TC）、低密度脂卵白胆固醇（LDL-C）、高密度脂卵白胆固醇（HDL-C）、去甲肾上腺素（NE）含量；称量腹部白色脂肪组织质料，给与苏木精-伊红染色法不雅察各组小鼠腹部白色脂肪组织情势改变；及时荧光定量PCR法检测小鼠下丘脑LKB1、AMPK及腹部脂肪组织过氧化物酶体增殖物激活受体γ共激活因子1α（PGC-1α）、解偶联卵白-1（UCP1）、过氧化物酶体增殖物激活受体γ（PPAR-γ）、β3-肾上腺素能受体（β3-AR）mRNA抒发；Western blot法检测小鼠下丘脑LKB1、AMPK和腹部白色脂肪组织PGC-1α、UCP1、PPAR-γ、β3-AR的卵白抒发。胁制：与普通组比较，模子组小鼠体质料，Lee’s指数，血清TC、TG、LDL-C含量升高（P<0.01），HDL-C含量质问（P<0.01），腹部白色脂肪组织质料增多（P<0.01），腹部白色脂肪组织脂肪细胞直径显着增大、单元面积细胞数量减少，血清NE含量及白色脂肪组织β3-AR、PGC-1α、UCP1、PPAR-γ的mRNA和卵白抒发质问（P<0.01），下丘脑LKB1、AMPK的mRNA和卵白抒发升高（P<0.01）。与模子组比较，电针组小鼠体质料，Lee’s指数，血清TC、TG、LDL-C含量均显赫质问（P<0.01），HDL-C含量升高（P<0.01），白色脂肪组织质料减少（P<0.01），腹部白色脂肪组织脂肪细胞直径减小、单元面积细胞数量增多，血清NE含量及白色脂肪组织β3-AR、PGC-1α、UCP1、PPAR-γ的mRNA和卵白抒发升高（P<0.01），下丘脑LKB1、AMPK的mRNA和卵白抒发质问（P<0.01）。论断：电针不错质问痴肥小鼠体质料、Lee’s指数，编削痴肥小鼠血脂水平，松开腹部白色脂肪组织质料，其机制可能与通过编削下丘脑LKB1/AMPK信号通路，激活脂肪组织交感神经活性，促进白色脂肪棕色化关联。

【Abstract】Objective To observe the effect of electroacupuncture (EA) on hypothalamic liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) signaling pathway, sympathetic nerve activity and browning of white adipose tissue (WAT) in obese mice, so as to explore the possible central mechanisms of EA in improving obesity. Methods C57BL/6J male mice were divided into normal group (n=8) and high fat diet group (n=30) randomly, and fed by normal diet and high fat diet for 8 weeks, respectively. In the high fat diet group, mice with body weight over 20% heavier than that of the normal group were considered as obese mice. The obese mice were divided into model and EA groups (n=8 in each group). Mice in the EA group were treated with EA (2 Hz/15 Hz, 1 mA, 3 mm needling depth) at “Zusanli” (ST36) and “Tianshu” (ST25) for 30 min, 5 times a week for 4 weeks. The mice’s body weight and length were measured for calculating the Lee’s index. The contents of serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and noradrenaline (NE) were detected by ELISA. The weight of abdominal white adipose tissue was weighed, and the morphological changes of abdominal adipose tissue were observed by HE staining. The mRNA and protein expression levels of LKB1 and AMPK in hypothalamus, peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), uncoupling protein-1 (UCP1), peroxisome proliferator-activated receptor γ (PPAR-γ) and β3-adrenergic receptor (β3-AR) in abdominal white adiposetissue were detected by real-time fluorescence quantitative PCR and Western blot, respectively. Result Compared with the normal group, the body weight, Lee’s index, serum contents of TC, TG and LDL-C, abdominal WAT mass, hypothalamus LKB1, AMPK mRNA and protein expression were increased (P<0.01), while serum HDL-C and NEcontents, mRNA and protein expression of β3-AR, PGC-1α, UCP1 and PPAR-γ in adipose tissue were decreased (P<0.01) in the model group. The diameter of adipocytes in abdominal WAT was significantly increased, and the number of cells per unit area was decreased in the model group. Following EA intervention, the body weight, Lee’s index, serum contents of TC, TG and LDL-C, abdominal WAT mass, hypothalamus LKB1, AMPK mRNA and protein expression were decreased (P<0.01), while the serum HDL-C and NE contents, mRNA and protein expression of β3-AR, PGC-1α, UCP1 and PPAR-γ were increased (P<0.01). The diameter of adipocytes in abdominal WAT was decreased and the number of cells per unit area was increased in the EA group. Conclusion EA can reduce the body weight and Lee’s index in obese mice, regulate blood lipids, and reduce the weight of abdominal WAT, which may be closely related to its effect in regulating the hypothalamic LKB1/AMPK signaling pathway, activating the adipose tissue sympathetic nerve activity, and promoting the browning of WAT.

06

基于转录组学技能商量基底外侧杏仁核参与电针调养癌痛偏抓诱发的抑郁厚谊的分子机制

Molecular mechanism of basolateral amygdala involved in electroacupuncture-induced amelioration of cancer pain and concomitant depression based on transcriptomics techniques

【摘抄】方针：不雅察电针对癌痛模子小鼠祸患偏抓诱发的抑郁厚谊的影响，给与转录组学技能从基因层面商量基底外侧杏仁核（BLA）参与癌痛多维度编削效应的分子机制。设施：将C57BL/6小鼠分为假手术组、模子组、电针组，每组10只。于股骨内打针含有Lewis肺癌细胞的PBS悬液建造骨癌痛模子。电针组小鼠在造模后第10天启动进行电针干涉“足三里”“三阴交”，逐日1次，每次20 min，连气儿干涉12 d。给与X线和HE染色不雅察荷瘤股骨远端骨质毁伤情况；von Frey检测小鼠机械性痛阈值变化；糖水偏好实验和将就拍浮实验不雅察小鼠抑郁厚谊；索取小鼠BLA组织RNA建库，转录组学测序筛选各异基因并进行生物信息学分析；及时荧光定量PCR检测各异抒发基因肿瘤坏死因子超家眷8（Tnfsf8）、骨髓基质细胞抗原1（Bst1）、前强啡肽原（Pdyn）、电压门控钠离子通说念β4（Scn4b）mRNA抒发水平。胁制：X线和HE染色可见小鼠股骨远端显赫骨质毁伤。与假手术组比较，模子组小鼠造模后第1、4、7、10、14、21天机械性痛阈值显赫质问（P<0.001，P<0.000 1），糖水偏好指数显赫质问（P<0.001），将就拍浮不动时辰显赫延长（P<0.001）。与模子组比较，电针组小鼠造模后第14、21天机械痛阈值显赫升高（P<0.000 1），糖水偏好指数显赫升高（P<0.05），将就拍浮不动时辰显赫裁减（P<0.01）。与假手术组比较，模子组小鼠BLA共筛选出404条各异基因，与模子组比较，电针组小鼠BLA共筛选出329条各异基因，其中在模子组抒发上调而在电针组下调的基因共45条，在模子组抒发相配上调而被电针干涉下调的基因共28条。将筛选的各异抒发基因进行基因施行（GO）防备和京齐基因组百科全书（KEGG）通路分析，GO防备胁制披露上述各异基因主邀功能富集在与神经炎性响应关连的配体受体活性、细胞因子受体结合及细胞因子活性，以及与神经元欢叫关连的神经肽信号通路、钙离子介导的信号传导等方面；KEGG通路分析披露各异基因主要富集在与炎性响应关连的通路如白细胞介素-17通路等。模子组Tnfsf8、Bst1 mRNA的抒发较假手术组显赫升高（P<0.01，P<0.05），电针组较模子组显赫下跌（P<0.01，P<0.05）；模子组Pdyn、Scn4b mRNA的抒发较假手术组显赫下跌（P<0.01），电针组较模子组显赫升高（P<0.05，P<0.01），及时荧光定量PCR胁制与基因测序胁制变化趋势相一致。论断：电针不错显赫缓解癌痛偏抓诱发的抑郁样厚谊，其机制可能与模子动物BLA内神经炎性响应的缓解及特定神经元的相配行为的规复关联。

【Abstract】Objective To observe the effect of electroacupuncture (EA) of “Zusanli” (ST 36) and “Sanyinjiao” (SP 6) on cancer pain and concomitant negative emotion in cancer pain model mice, and to explore its molecular mechanisms in the basolateral amygdala (BLA) by using transcriptomics techniques. Methods C57BL/6 mice were randomized into sham operation, model and EA groups, with 10 mice in each group. The cancer pain model was established by injecting PBS suspension containing Lewis lung cancer cells into the femur. The mice in the EA group received EA stimulation (1 mA, 2 Hz) on ST 36 and SP 6 from the 10th day after modeling, 20 min per day for 12 successive days. The bone damage of the distal femur was observed with X-ray and H.E. staining, respectively. The mechanical pain threshold (MPT) was detected by using von Frey. The depression-like behavior was detected by using sucrose-preference test (sucrose preference index in 12 h), and the immobility (feeling of despair) duration of forced swimming within 4 min. The BLA tissue was extracted for RNA sequencing (RNA library construction, and screening differential gene profiling by transcriptomic sequencing) and bioinformatics analysis. The real-time PCR was used to validate the mRNA expression of differentially expressed genes: tumor necrosis factor superfamily 8 (Tnfsf8), bone marrow stromal cell antigen 1 (Bst1), prodynorphin (Pdyn) and voltage-gated sodium channelβ4 (Scn4b). Results H.E. staining and X-ray showed significant bone damage in the distal femur in cancer pain mice. In contrast to the sham operation group, the MPT on the 1st, 4th,7th, 10th, 14th and 21st day after modeling and sucrose preference index were significantly decreased (P<0.001, P<0.000 1), and the immobility time of the forced swimming was considerably increased in the model group (P<0.001). In contrast to the model group, the MPT values on the 14th and 21st day and sucrose preference index were obviously increased (P<0.000 1, P<0.05), and the immobility time was strikingly decreased in the EA group (P<0.01). RNA sequencing showed that a total of 404 differentially expressed genes (205 up-regulated, 199 down-regulated) were screened in the model group compared with the sham operation group, and a total of 329 differentially expressed genes (206 up-regulated and 123 down-regulated) were screened in the EA group compared with the model group. Venn diagram analysis of the differentially expressed genes showed that 45 up-regulated and 28 down-regulated genes in the model group were completely reversed by EA. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the screened differentially expressed genes revealed that the above differential genes were mainly enriched in the ligand receptor activity, cytokine receptor binding, and cytokine activity related to neuro-inflammation, as well as in neuropeptide signaling pathways related to neuronal excitability, and calcium ion mediated signal transduction. The analysis of KEGG pathway showed that the differentially expressed genes were mainly enriched in the inflammation-related pathways, such as interleukin-17 pathway. Validation analysis of the differentially expressed genes showed that the expression levels of Tnfsf8 and Bst1 were significantly up-regulated in the model group compared with the sham operation group (P<0.01, P<0.05), and down- regulated by EA (P<0.01, P<0.05), while the expression levels of Pdyn and Scn4b were down-regulated in the model group in comparison with the sham operation group (P<0.01), and up-regulated by EA (P<0.05, P<0.01), which was consistent with the changing trend of the gene sequencing results. Conclusion Acupuncture of ST36 and SP6 can significantly relieve cancer pain and concomitant negative emotion in cancer pain mice, which may be related to its functions in alleviating neuro-inflammation and relieving the abnormal activities of specific neurons in the BLA.

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